454 Life Sciences: Record Setting 6.25 MPH (Million bases Per Hour)

The proliferation of large-scale DNA-sequencing projects in recent years has driven a search for alternative methods to reduce time and cost. Here we describe a scalable, highly parallel sequencing system with raw throughput significantly greater than that of state-of-the-art capillary electrophoresis instruments. The apparatus uses a novel fibre-optic slide of individual wells and is able to sequence 25 million bases, at 99% or better accuracy, in one four-hour runan approximately 100-fold increase in throughput over current Sanger sequencing technology

This from the paper’s abstract.

Marcel Margulies et al. "Genome sequencing in microfabricated high-density picolitre reactors" Nature (online). July 31 2005. (subscription only)

Nick Wade. "DNA machine may advance genetic sequencing for patients" NYTimes. August 1, 2005.

Kevin Davies, "Fantastic 454" Bio-IT World. August 1, 2005.


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